Published: 08 September, 2021 | Volume 5 - Issue 2 | Pages: 089-093
Figure 2:
Eff ects of MLL-1 inhibition on K+ and Na+ currents in diff erentiated NS20Y cells. The left panels show sample traces for measurements of K+ (a), Na+ (b), and KIR (c) channels, respectively. The right panels demonstrate: (a) Steady-state K+ channel current density with voltage steps from -50 to +100 mV, which is indicative of Kv type of K+ channels. MLL-1 inhibition (open circles) signifi cantly increased steady-state current density in main eff ect (F(1, 24) = 8.429, p = 0.0078) in a voltage dependent manner (interaction of MLL-1 inhibition X Voltage, F(15, 360) = 6.436, p < 0.0001), with significance from +50 to +100 mV, inclusive (+50 mV, p = 0.0272; +60 mV, p = 0.0036, +70 mV, p = 0.0004, +80 and +90 mV, p < 0.0001; +100 mV, p = 0.0001) when
analyzed with two-way ANOVA. (b) Na+ channel current density (normalized with cell size) in response to voltage steps from -50 to +70 mV. MLL-1 inhibition (open circles) significantly decreased the peak current density in a voltage-dependent manner (main eff ect, F(1,17) = 15.37, p = 0.0011; interaction of MLL-1 inhibition X Voltage, F(18, 306) = 7.931, p < 0.0001; -20 to +10 mV, p < 0.0001; +20 mV, p = 0.0002; +30 mV, p = 0.0013; +40 mV, p = 0.0087; +50 mV, p = 0.0416). (c) Peak K+ channel current density with voltage steps from -60 to -140 mV, which is indicative of the KIR type K+ channel, and for which MLL-1 inhibition did not result in changes in current density (main eff ect, F(1, 24) = 0.9399, p = 0.3420; interaction of MLL-1 inhibition X Voltage, F(8,192) = 0.03859, p = 0.9999). Numbers in parentheses indicate the total numbers of cells analyzed; cells were collected from at least two independent cultures.
Read Full Article HTML DOI: 10.29328/journal.jnnd.1001057 Cite this Article Read Full Article PDF
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