Published: 08 September, 2021 | Volume 5 - Issue 2 | Pages: 089-093
Figure 1:
MLL-1 inhibition-induced changes in cellular levels of H3K4me3 and ion channel proteins. Differentiated NS20Y cells were treated with either 100 uM MM-102 (Inhib) or vehicle (0.1% DMSO, Ctrl). ICC analysis of H3K4me3 (a) or Kv2.1 (b) or Nav1.2 (c) was performed using appropriate primary antibodies and Dylight 594-conjugated secondary antibodies (a and b, anti-mouse; c, anti-rabbit). A negative control (d, Minus
primary) was included by incubating the specimen with secondary antibody alone in absence of the primary antibody. After incubation with antibodies, the specimens were mounted with a DAPI-containing solution to reveal nuclei, and examined under a fl uorescence microscope. Similar results were obtained in 2-3 independent cultures. Scale bar represents 50 μm.
Read Full Article HTML DOI: 10.29328/journal.jnnd.1001057 Cite this Article Read Full Article PDF
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